Engineering enzyme conformation within liquid-solid hybrid microreactors for enhanced continuous-flow biocatalysis.

ABSTRACT: The artificial engineering of an enzyme's structural conformation and dynamic properties to promote its catalytic activity and stability outside cellular environments is highly pursued in industrial biotechnology. Here, we describe an elegant strategy of combining the rationally designed liquid-solid hybrid microreactor with a tailor-made polyethylene glycol functional ionic liquid (PEG-IL) microenvironment to exercise a high level of control over the configuration of enzymes for practical continuous-flow biocatalysis. As exemplified by a lipase driven kinetic resolution reaction, the obtained system exhibits a 2.70 to 30.35-fold activity enhancement compared to their batch or traditional IL-based counterparts. Also, our results demonstrate that the thermal stability of encapsulated lipase

SUBMITTER: Hao X 

PROVIDER: S-EPMC11608221 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature