Subconjunctival aflibercept inhibits corneal angiogenesis and VEGFR-3⁺CD11b⁺ cells.

ABSTRACT:

Purpose

This study aimed to investigate the effects of subconjunctival injection of aflibercept, a soluble protein decoy for VEGFR-1 and VEGFR-2, on corneal angiogenesis and VEGFR-expressing CD11b⁺ cells in a mouse model of suture-induced corneal neovascularization.

Methods

Corneal neovascularization was induced in BALB/c mice by placing three sutures on the cornea. Immediately after surgery, either 200 µg aflibercept (5 µL) or an equal volume of phosphate-buffered saline (PBS) was administered into the subconjunctival space. Seven days after later, corneal new vessels were quantified through clinical examination and measurement of the CD31-stained area in corneal flat mounts. The levels of pro-angiogenic and inflammatory markers in the cornea were evaluated using

SUBMITTER: Yoon CH 

PROVIDER: S-EPMC11608285 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature