Ectopic USP15 expression inhibits HIV-1 transcription involving changes in YY1 deubiquitination and stability.

ABSTRACT:

Introduction

Protein homeostasis is maintained by the opposing action of ubiquitin ligase and deubiquitinase, two important components of the ubiquitin-proteasome pathway, and contributes to both normal physiological and pathophysiological processes. The current study aims to delineate the roles of ubiquitin-specific protease 15 (USP15), a member of the largest deubiquitinase family, in HIV-1 gene expression and replication.

Methods

We took advantage of highly selective and specific ubiquitin variants (UbV), which were recently designed and developed for USP15, and ascertained the inhibitory effects of USP15 on HIV-1 gene expression and production by transfection and Western blotting. We also used real-time RT-PCR, transcription factor profiling, subcellular fractionation, i

SUBMITTER: Rezaei S 

PROVIDER: S-EPMC11609158 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature