Response and adaptation of the transcriptional heat shock response to pressure.

ABSTRACT:

Introduction

The molecular mechanisms underlying pressure adaptation remain largely unexplored, despite their significance for understanding biological adaptation and improving sterilization methods in the food and beverage industry. The heat shock response leads to a global stabilization of the proteome. Prior research suggested that the heat shock regulon may exhibit a transcriptional response to high-pressure stress.

Methods

In this study, we investigated the pressure-dependent heat shock response in E. coli strains using plasmid-borne green fluorescent protein (GFP) promoter fusions and fluorescence fluctuation microscopy.

Results

We quantitatively confirm that key heat shock genes-rpoH, rpoE, dnaK, and groEL - are transcriptiona

SUBMITTER: Coffin CH 

PROVIDER: S-EPMC11609203 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature