Development and characterization of high-affinity aptamers for HIV protease detection.

ABSTRACT:

Background

There are 39 million people infected by the human immunodeficiency virus (HIV) and 1.3 million new annually infections with more than 150 variants circulating. Early HIV detection is crucial for timely antiretroviral therapy and transmission prevention, but no technique can detect HIV before 10 days of infection. Aptamers, single-stranded oligonucleotides, can recognize specific proteins with high affinity and specificity. New molecular assays based on aptamers selected against highly-conserved peptides in HIV proteins could help to detect the virus earlier.

Results

We have selected and characterized the first two aptamers against a highly-conserved and exposed peptide in the HIV protease (PR) after analyzing 236,240/6,436 HIV-1/HIV-2 PR sequences of nearly 150 HI

SUBMITTER: Valades-Alcaraz A 

PROVIDER: S-EPMC11609250 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature