AAV delivery strategy with mechanical support for safe and efficacious cardiac gene transfer in swine.

ABSTRACT: Adeno-associated virus-based gene therapy is a promising avenue in heart failure treatment, but has shown limited cardiac virus uptake in humans, requiring new approaches for clinical translation. Using a Yorkshire swine ischemic heart failure model, we demonstrate significant improvement in gene uptake with temporary coronary occlusions assisted by mechanical circulatory support. We first show that mechanical support during coronary artery occlusions prevents hemodynamic deterioration (n = 5 female). Subsequent experiments show that coronary artery occlusions during gene delivery improve gene transduction, while adding coronary sinus occlusion (Stop-flow) further improves gene expression up to >1 million-fold relative to conventional intracoronary infusion. Complete survival during and af

SUBMITTER: Mazurek R 

PROVIDER: S-EPMC11609281 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature