Physicochemical and biological impacts of light stress on adeno-associated virus serotype 6.

ABSTRACT: Recombinant adeno-associated virus (rAAV) is a small, nonenveloped virus vector that has attracted attention as a gene therapy platform. Several studies have revealed the stability characteristics of rAAV; however, its tolerance against light stress remains unclear. Here, the physicochemical and biological impacts of light stress on rAAV6 with EGFP as a gene of interest were investigated. The results revealed that light exposure led to an approximately 20% loss in particle number of rAAV with s value of full particles, whereas a more drastic decrease was observed in the genomic titer due to the degradation of encapsidated DNA, leading to an approximately 90% reduction in biological activity as evident from a flow cytometry-based assay. Structure analysis of the viral proteins VP1, V

SUBMITTER: Takino R 

PROVIDER: S-EPMC11609365 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature