Glutathione has cell protective and anti-catabolic effects in articular cartilage without impairing the chondroanabolic phenotype.

ABSTRACT: Joint injuries and consequent oxidative stress is a high-risk factor for developing post-traumatic osteoarthritis (OA). While antioxidative therapy using N-acetylcysteine (NAC) has cell- and chondroprotective effects following cartilage injury, it strongly impairs matrix synthesis. Consequently, direct application of Glutathione (GSH) was tested as an alternative therapeutic approach using an ex vivo cartilage trauma model and isolated chondrocytes, with comparison to NAC. Porcine cartilage explants were traumatized using a drop tower with an impact energy of 0.47 J and afterwards treated with 0.5-2 mM GSH or 2 mM NAC for 4 days according to a standardized protocol. The effects of antioxidative treatment on the chondrogenic phenotype were tested in a 3D pellet culture for 28 days. O

SUBMITTER: Maurer S 

PROVIDER: S-EPMC11609657 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature