Disruption of bioenergetics enhances the radio-sensitivity of patient-derived glioblastoma tumorspheres.

ABSTRACT:

Background

Despite available treatment approaches, including surgical resection along with chemotherapy and radiotherapy, glioblastoma (GBM), the most prevalent primary brain tumor, remains associated with a grim prognosis. Although radiotherapy is central to GBM treatment, its combination with bioenergetics regulators has not been validated in clinical practice. Here, we hypothesized that bioenergetics regulators can enhance the radio-sensitivity of GBM tumorspheres (TSs).

Methods

Gene expression profiles of GBM patient-derived TSs were obtained through microarray and RNA-seq. In vitro treatment efficacy was assessed using clonogenic assay, 3D invasion assay, neurosphere formation assay, and flow cytometry. Protein expression was measured via western blot, and γH2AX foci we

SUBMITTER: Kim SJ 

PROVIDER: S-EPMC11609692 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature