Potential differences in receptor-mediated G-protein activation in postmortem human hippocampal membranes prepared from healthy controls and suicide victims.

ABSTRACT:

Aim

Postmortem brain studies offer enormous opportunities to study molecular mechanisms associated with suicide. In the present study, conventional [³⁵S]GTPγS binding assay and its version-up method ([³⁵S]GTPγS binding/immunoprecipitation assay) were applied to postmortem human hippocampal membranes prepared from suicide victims and control subjects.

Methods

By using conventional [³⁵S]GTPγS binding assay, functional activations of G_i/o proteins coupled with multiple GPCRs (5-HT_1A receptor, α_2A-adrenoceptor, M₂/M₄ mAChRs, adenosine A₁ receptor, histamine H₃ receptor, group II mGlu, GABA_B receptor, μ-opioid receptor, δ-opioid receptor, and NOP receptor) were

SUBMITTER: Odagaki Y 

PROVIDER: S-EPMC11609751 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature