Resistance characterization and transcriptomic analysis of imipenem-induced drug resistance in <i>Escherichia coli</i>.

ABSTRACT:

Background

Bacteria can develop resistance to various antibiotics under selective pressure, leading to multifaceted changes in resistance mechanisms. Transcriptomic sequencing allows for the observation of transcriptional level alterations in cells under antibiotic stress. Understanding the bacterial response to such stress is essential for deciphering their strategy against drug-resistant antibiotics and identifying potential targets for antibiotic development.

Methods

This study using wild-type (WT) Escherichia coli (E. coli) discovered that continuous in vitro induction screening for imipenem-resistant strains resulted in bacteria with enhanced biofilm-forming ability and mutations in antibiotic target sites. Transcriptomic sequencing of the resistant

SUBMITTER: Tong C 

PROVIDER: S-EPMC11610472 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature