Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery.

ABSTRACT: Heavy chain-only antibodies have found many applications where conventional heavy-light heterodimeric antibodies are not favorable. Heavy chain-only antibodies with their single antigen-binding domain offer the advantage of a smaller size and higher stability relative to conventional antibodies, and thus, the potential for novel targeting modalities. Domain antibodies have commonly been sourced from camelids with ex-vivo humanization or transgenic rodents expressing heavy chains without light chains, but these host species are all mammalian, limiting their capacity to elicit robust immune responses to conserved mammalian targets. We have developed transgenic chickens expressing heavy chain-only antibodies with a human variable region to combine the superior target recognition advant

SUBMITTER: Vuong CN 

PROVIDER: S-EPMC11610561 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature