Long-read Ribo-STAMP simultaneously measures transcription and translation with isoform resolution.

ABSTRACT: Transcription and translation are intertwined processes in which mRNA isoforms are crucial intermediaries. However, methodological limitations in analyzing translation at the mRNA isoform level have left gaps in our understanding of critical biological processes. To address these gaps, we developed an integrated computational and experimental framework called long-read Ribo-STAMP (LR-Ribo-STAMP) that capitalizes on advancements in long-read sequencing and RNA-base editing-mediated technologies to simultaneously profile translation and transcription at both the gene and mRNA isoform levels. We also developed the EditsC metric to quantify editing and leverage the single-molecule, full-length transcript information provided by long-read sequencing. Here, we report concordance between gene-lev

SUBMITTER: Jagannatha P 

PROVIDER: S-EPMC11610582 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature