Challenges in identifying mRNA transcript starts and ends from long-read sequencing data.

ABSTRACT: Long-read sequencing (LRS) technologies have the potential to revolutionize scientific discoveries in RNA biology through the comprehensive identification and quantification of full-length mRNA isoforms. Despite great promise, challenges remain in the widespread implementation of LRS technologies for RNA-based applications, including concerns about low coverage, high sequencing error, and robust computational pipelines. Although much focus has been placed on defining mRNA exon composition and structure with LRS data, less careful characterization has been done of the ability to assess the terminal ends of isoforms, specifically, transcription start and end sites. Such characterization is crucial for completely delineating full mRNA molecules and regulatory consequences. However, there are

SUBMITTER: Calvo-Roitberg E 

PROVIDER: S-EPMC11610588 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature