Homonuclear Super-Resolution NMR Spectroscopy.

ABSTRACT: In homonuclear ¹H NMR (nuclear magnetic resonance) spectra such as [¹H,¹H]-NOESY (Nuclear Overhauser Enhancement spectroscopy), which is a historic cornerstone spectrum for biomolecular NMR structural biology, hundreds to thousands of cross peaks are present within a square of approximately 100 ppm² leading to a lot of signal overlap. Spectral resolution is thus a limiting factor for unambiguous chemical shift assignment and data interpretation for dynamics and structure elucidation. Acquiring the spectra at higher magnetic fields such as at a 1.2 GHz ¹H frequency helps to reduce spectral crowding, since resolution scales proportionally to the magnetic field strength. Here, we show that the linewidths of cross peaks in [¹H,<

SUBMITTER: Gampp O 

PROVIDER: S-EPMC11610657 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature