NMR Screen Reveals the Diverse Structural Landscape of a G-Quadruplex Library.

ABSTRACT: G-quadruplexes are noncanonical nucleic acid structures formed by stacked guanosine tetrads. Despite their functional and structural diversity, a single consensus model is typically used to describe sequences with the potential to form G-quadruplex structures. We are interested in developing more specific sequence models for G-quadruplexes. In previous work, we functionally characterized each sequence in a 496-member library of variants of a monomeric reference G-quadruplex for the ability to bind GTP, promote a model peroxidase reaction, generate intrinsic fluorescence, and to form multimers. Here we used NMR to obtain a broad overview of the structural features of this library. After determining the ¹H NMR spectrum of each of these 496 sequences, spectra were sorted into multi

SUBMITTER: Sgallova R 

PROVIDER: S-EPMC11610706 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature