Transplanted MSCs promote alveolar bone repair via hypoxia-induced extracellular vesicle secretion.

ABSTRACT:

Object

Mesenchymal stem cell (MSC) therapy is a potential strategy for promoting alveolar bone regeneration. This study evaluated the effects and mechanisms of transplanted MSCs on alveolar bone repair.

Methods

Mouse alveolar bone defect model was treated using mouse bone marrow mesenchymal stem cell (BMSC) transplantation. The bone repair was evaluated by micro-CT and Masson staining. The conditioned medium of hypoxia-treated BMSCs was co-cultured with normal BMSCs in vitro to detect the regulatory effect of transplanted MSCs on the chemotactic and migratory functions of host cells. The mechanisms were investigated using Becn siRNA transfection and western blotting.

Results

BMSC transplantation promoted bone defect regeneration. The hypoxic microenvironment induces B

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11610711 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature