O-methyltransferase CbzMT catalyzes iterative 3,4-dimethylations for carbazomycin biosynthesis.

ABSTRACT: Carbazomycins (1-8) are a subgroup of carbazole derivatives that contain oxygen at the C3 and C4 positions and an unusual asymmetric substitution pattern. Several of these compounds exhibit antifungal and antioxidant activities. To date, no systematic biosynthetic studies have been conducted on carbazomycins. In this study, carbazomycins A and B (1 and 2) were isolated from Streptomyces luteosporeus NRRL 2401 using a one-strain-many-compound (OSMAC)-guided natural product mining screen. A biosynthetic gene cluster (BGC) was identified, and possible biosynthetic pathways for 1 and 2 were proposed. The in vivo genetic manipulation of the O-methyltransferase-encoding gene cbzMT proved indispensable for 1 and 2 biosyn

SUBMITTER: Lin B 

PROVIDER: S-EPMC11610953 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature