Identification of multiple regulatory genes involved in TGase production in Streptomyces mobaraensis DSM 40587.

ABSTRACT: Microbial transglutaminase (TGase) is a protein that is secreted in a mature form and finds wide applications in meat products, tissue scaffold crosslinking, and textile engineering. Streptomyces mobaraensis is the only licensed producer of TGase. However, increasing the production of TGase using metabolic engineering and heterologous expression approaches has encountered challenges in meeting industrial demands. Therefore, it is necessary to identify the regulatory networks involved in TGase biosynthesis to establish a stable and highly efficient TGase cell factory. In this study, we employed a DNA-affinity capture assay and mass spectrometry analysis to discover several transcription factors. Among the candidates, eight were selected and found to impact TGase biosynthesis. Notably

SUBMITTER: Liu X 

PROVIDER: S-EPMC11611014 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature