Constitutive overexpression of cellobiohydrolase 2 in <i>Trichoderma reesei</i> reveals its ability to initiate cellulose degradation.

ABSTRACT: Cellulose degradation results from the synergistic effect of different enzymes, but which enzyme is involved in the initial stage of cellulose degradation is still not well understood. Cellobiohydrolase 2 (CBH2) attached to the conidial surface is possibly associated with the initial stage. However, its specific mechanism is still incompletely known. This study explored the potential role of CBH2 in initiating cellulose degradation using a constitutive overexpression strategy. First, the CBH2-overexpression Trichoderma reesei strains Qgc2-5 and Qrc2-40 were constructed using the constitutive promoters Pgpd1 and PrpS30, respectively. It was found that cbh2 was expressed at a high level under the glucose conditions and was significantly higher than that of the par

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11611025 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature