Metagenomics insights into bacterial diversity and antibiotic resistome of the sewage in the city of Belem, Para, Brazil.

ABSTRACT:

Introduction

The advancement of antimicrobial resistance is a significant public health issue today. With the spread of resistant bacterial strains in water resources, especially in urban sewage, metagenomic studies enable the investigation of the microbial composition and resistance genes present in these locations. This study characterized the bacterial community and antibiotic resistance genes in a sewage system that receives effluents from various sources through metagenomics.

Methods

One liter of surface water was collected at four points of a sewage channel, and after filtration, the total DNA was extracted and then sequenced on an NGS platform (Illumina® NextSeq). The sequenced data were trimmed, and the microbiome was predicted using the Kraken software, while the re

SUBMITTER: Ramos S 

PROVIDER: S-EPMC11611572 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature