Allele-tagged TaqMan^® PCR genotyping assays for high-throughput detection of soybean cyst nematode resistance.

ABSTRACT:

Background

Whole genome resequencing (WGRS) platforms provide exceptional fingerprinting of the entire genome but are expensive and less flexible to use as a routine genotyping tool for targeting causal polymorphisms within a germplasm collection or breeding program. Therefore, there has been a continuous effort to develop small-scale genotyping platforms that facilitate robust and quick assessments of the allelic status of causal variants for important traits within soybean breeding programs. The objective was to develop a comprehensive panel of soybean cyst nematode (SCN) resistance TaqMan^® assays via selecting the causative genes and analyzing their associated alleles.

Methods

The Soybean Allele Catalog was utilized to investigate WGRS-derived variants which ar

SUBMITTER: Usovsky M 

PROVIDER: S-EPMC11611941 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature