Identification of reference genes for gene expression assessment in Avena sativa under biotic stress triggered by Blumeria graminis.

ABSTRACT: A repeatable and reliable reverse transcription quantitative PCR (RT-qPCR) experiment depends upon proper reference genes (RGs) selection. This study aims to examine the expression stability of nine candidate RGs for the Avena sativa - Blumeria graminis experimental setup. B. graminis causes powdery mildew - the most devastating and economically important fungal disease of crops worldwide. RGs were evaluated in Pm3 and Pm4 oat differential lines and the susceptible cultivar Fuchs during compatible and incompatible interactions with different pathotypes of Blumeria graminis f. sp. avenae in six-time points post inoculation. The identification of genes exhibiting high expression stability was done by four algorithms (geNorm, NormFinder, BestKeeper and deltaCt). The results indicated that reg

SUBMITTER: Sowa S 

PROVIDER: S-EPMC11612269 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature