Biofilm-based biocatalysis for β-cyclodextrin production by the surface-display of β-cyclodextrin glycosyltransferase in Bacillus subtilis.

ABSTRACT: β-cyclodextrin (β-CD) is an important cyclic oligosaccharide, which is widely applicated in foods, environmental protection, and cosmetics, primarily prepared from enzymatic synthesis in traditional industry. However, several challenges persist, including cumbersome processes and difficulties in achieving continuous fermentation and catalysis. This research introduced a biofilm-based immobilized fermentation, integrating with enzyme catalysis system of surface display in Bacillus subtilis. The bslA gene was selected to construct the surface display system due to its ability to promote biofilm formation and serve as an anchorin. Compared to free cell catalysis, the biofilm-based immobilized catalysis expanded the temperature range to 40-70 and the pH range to 5-7.5. During the continuous ca

SUBMITTER: Wang D 

PROVIDER: S-EPMC11612409 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature