Loss of neuropeptide signalling alters temporal expression of mouse suprachiasmatic neuronal state and excitability.

ABSTRACT: Individual neurons of the hypothalamic suprachiasmatic nuclei (SCN) contain an intracellular molecular clock that drives these neurons to exhibit day-night variation in excitability. The neuropeptide vasoactive intestinal polypeptide (VIP) and its cognate receptor, VPAC₂, are synthesized by SCN neurons and this intercellular VIP-VPAC₂ receptor signal facilitates coordination of SCN neuronal activity and timekeeping. How the loss of VPAC₂ receptor signalling affects the electrophysiological properties and states of SCN neurons as well as their responses to excitatory inputs is unclear. Here we used patch-clamp electrophysiology and made recordings of SCN neurons in brain slices prepared from transgenic animals that do not express VPAC₂ receptors (

SUBMITTER: Wegner S 

PROVIDER: S-EPMC11612845 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature