Behavioural phenotypes of Dicer knockout in the mouse SCN.

ABSTRACT: The suprachiasmatic nucleus (SCN) is the master clock that directly dictates behavioural rhythms to anticipate the earth's light/dark cycles. Although post-transcriptional regulators called microRNAs have been implicated in physiological SCN function, how the absence of the entire mature miRNome impacts SCN output has not yet been explored. To study the behavioural consequences of miRNA depletion in the SCN, we first generated a mouse model in which Dicer is inactivated in the SCN by crossing Syt10^Cre mice with Dicer^flox mice to study behavioural consequences of miRNA depletion in the SCN. Loss of all mature miRNAs in the SCN shortened the circadian period length by ~37 minutes at the tissue level and by ~45 minutes at the locomotor activity level. Moreover, knockout

SUBMITTER: Du NH 

PROVIDER: S-EPMC11612849 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature