Polymorphic potential of SRF binding site of c-Fos gene promoter: in vitro study.

ABSTRACT: Recently published in vivo observations have highlighted the presence of cruciform structures within the genome, suggesting their potential significance in the rapid recognition of the target sequence for transcription factor binding. In this in vitro study, we investigate the organization and stability of the sense (coding) strand within the Serum Response Element of the c-Fos gene promoter (c-Fos SRE), specifically focusing on segments spanning 12 to 36 nucleotides, centered around the CArG-box. Through a thorough examination of UV absorption patterns with varying temperatures, we identified the emergence of a remarkably stable structure, which we conclusively characterized as a hairpin using complementary ¹H NMR experiments. Our research dec

SUBMITTER: Profantova B 

PROVIDER: S-EPMC11613138 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature