A Single-Cell Interrogation System from Scratch: Microfluidics and Deep Learning.

ABSTRACT: Live-cell imaging using fluorescence microscopy enables researchers to study cellular processes in unprecedented detail. These techniques are becoming increasingly popular among microbiologists. The emergence of microfluidics and deep learning has significantly increased the amount of quantitative data that can be extracted from such experiments. However, these techniques require highly specialized expertise and equipment, making them inaccessible to many biologists. Here we present a guide for microbiologists, with a basic understanding of microfluidics, to construct a custom-made live-cell interrogation system that is capable of recording and analyzing thousands of bacterial cell-cycles per experiment. The requirements for different microbiological applications are varied, and experiment

SUBMITTER: Ripandelli RAA 

PROVIDER: S-EPMC11613446 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature