Application of UHPLC-ESI-MS/MS to Identify Free Radicals via Spin Trapping with BMPO.

ABSTRACT: Free radicals play an important role in many chemical and biological processes, but due to their highly reactive and short-lived nature, they evade most analytical techniques, limiting our understanding of their formation and reactivity. Spin trapping molecules can react with free radicals to form radical adducts with lifetimes long enough for analysis. Mass spectrometry is an attractive way to identify radical adducts, but due to their radical nature, they form untraditional oxidized [M]⁺ and reduced [M+2H]⁺ ions, which complicates the interpretation of mass spectrometry analysis. This work uses simplified mixtures of radicals generated in both water and dimethyl sulfoxide (DMSO) with spin trap 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), to eluc

SUBMITTER: Gerritz L 

PROVIDER: S-EPMC11613549 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature