SENP3 mediates deSUMOylation of SIX1 to promote prostate cancer proliferation and migration.

ABSTRACT:

Background

Sentrin/SUMO-specific protease 3 (SENP3) is essential to regulate protein stability and function in normal and cancer cells. Nevertheless, its role and action mechanisms in prostate cancer (PCa) remain elusive. Thus, clarification of SENP3's involvement and the SUMOylation process in PCa is pivotal for discovering potential targets and understanding SUMOylation dynamics.

Methods

Cell viability, EdU staining, live cell imaging, and cell cycle assays were used to determine proliferation of PCa cells. Transwell and wound-healing assays were used to detect migration of PCa cells. The interaction between SENP3 and SIX1 was determined by co-immunoprecipitation, western blotting, and immunofluorescence assays. Xenograft models established on NOD-SCID mice were used to ev

SUBMITTER: Shao Z 

PROVIDER: S-EPMC11613746 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature