Cross-species single-cell analysis reveals divergence and conservation of peripheral blood mononuclear cells.

ABSTRACT:

Background

Single-cell transcriptome sequencing (scRNA-seq) has revolutionized the study of immune cells by overcoming the limitations of traditional antibody-based identification and isolation methods. This advancement allows us to obtain comprehensive gene expression profiles from a diverse array of vertebrate species, facilitating the identification of various cell types. Comparative immunology across vertebrates presents a promising approach to understanding the evolution of immune cell types. In this study, we conducted a comparative transcriptome analysis of peripheral blood mononuclear cells (PBMCs) at the single-cell level across 12 species.

Results

Our findings shed light on the cellular compositional features of PBMCs, spanning from fish to mammals. Notably, we ide

SUBMITTER: Zhang S 

PROVIDER: S-EPMC11613757 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature