Characterization of the Retinal Circulation of the Mouse.

ABSTRACT:

Purpose

Mice are highly used in retinal research because, like humans, mice have vascularized retinas and choroidal circulation. Although the retinal circulation has been well-characterized in development, its stability during adulthood is less understood. To examine this network, we quantified several key metrics of the trilaminar vasculature.

Methods

We used mice (n = 15) with transgenic fluorescent NG2-DsRed (JX: #00824), a vascular-associated label in the retina. One eye per mouse was imaged using confocal microscopy (Nikon A1 Ti2 Eclipse) and traced with ImageJ SNT tools. Using an adaptive optics scanning light ophthalmoscope, additional mice (n = 3) were imaged at single-cell resolution within the living eye to measure the same vasculature.

Results

Across mice,

SUBMITTER: Shang F 

PROVIDER: S-EPMC11613998 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature