Inhibitory effect of Zhujing Pill on myopia progression: Mechanistic insights based on metabonomics and network pharmacology.

ABSTRACT:

Objectives

This study endeavored to uncover the mechanisms by which Zhujing pill (ZJP) slows myopia progression.

Methods

We employed biometric analyses to track diopter and axial length changes in guinea pigs with negative lens-induced myopia (LIM). Through integrating metabonomics and network pharmacology, we aimed to predict the anti-myopic targets and active ingredients of ZJP. Subsequent analysis, including real-time fluorescent quantitative PCR (qPCR) and Western blotting (WB), assessed the expression levels of CHRNA7, LPCAT1, and NOS2 in retinal tissues.

Key findings

Our findings demonstrate that ZJP significantly mitigates diopter increase and axial elongation in LIM guinea pigs. Metabonomic analysis revealed significant changes in 13 serum metabolites, with ZJ

SUBMITTER: Du Y 

PROVIDER: S-EPMC11614212 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature