Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method.

ABSTRACT: To use phages in a personalized therapy and industrial applications, an accurate quantification is needed. The gold standard method, namely the culture-based double agar overlay (DAO) method, provides an accurate estimate of the number of infectious phages but is laborious and time-intensive. Quantitative polymerase chain reaction (qPCR) can be used as a fast alternative but tends to overestimate the number of infectious phage particles. Here we describe the use of a DNase treatment before quantification of the Staphylococcus aureus phage ISP with qPCR to obtain a more accurate estimate of the number of infectious phage particles. We showed that DNase treatment results in a significant decrease of the concentration when measured with qPCR although for two out of three tested ISP phage stoc

SUBMITTER: Van Overfelt S 

PROVIDER: S-EPMC11614264 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature