Laser Aggregometry Assessment of Blood Microrheology in a Slit Fluidic Channel Covered With Endothelial Cells.

ABSTRACT: The blood rheology in vitro in glass or plastic microfluidic chips is different from that in vivo in blood vessels with similar geometry. Absence of vascular endothelium is suggested to cause these discrepancies. This work aims to perform in vitro measurements of blood microrheologic parameters in a slit microfluidic channel covered with endothelial cells (HUVEC). The laser aggregometry was employed to measure the intensity of laser light, backscattered from the blood flow, as a function of shear stress to evaluate the hydrodynamic strength of red blood cells (RBC) aggregates in terms of critical shear stress (CSS). The results demonstrated a decrease in CSS accompanied by an increase in the accuracy of its measurement at similar shear stresses when endothelial cells were present in the ch

SUBMITTER: Semenov AN 

PROVIDER: S-EPMC11614562 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature