Complex patterns of WNV evolution: a focus on the Western Balkans and Central Europe.

ABSTRACT:

Introduction

West Nile Virus, an emerging zoonotic pathogen, has been circulating in Serbia for over a decade, with its first detection in mosquitoes in 2010. Since then, the virus has led to increasing cases in both animals and humans, peaking in 2018 with 415 human cases and 36 fatalities. This study aimed to explore the phylogenetic relationships between previously sequenced West Nile virus strains from Serbia and those sequenced in this study, while also identifying possible virulence factors.

Materials and methods

Whole genome sequencing was conducted using a targeted approach on the MinION Mk1C platform, following a two-step process involving cDNA synthesis and amplification. Bioinformatics analysis included demultiplexing, primer trimming, and sequence mapping using t

SUBMITTER: Solaja S 

PROVIDER: S-EPMC11614783 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature