Optimising in-cell NMR acquisition for nucleic acids.

ABSTRACT: Understanding the structure and function of nucleic acids in their native environment is crucial to structural biology and one focus of in-cell NMR spectroscopy. Many challenges hamper in-cell NMR in human cell lines, e.g. sample decay through cell death and RNA degradation. The resulting low signal intensities and broad line widths limit the use of more complex NMR experiments, reducing the possible structural and dynamic information that can be extracted. Here, we optimize the detection of imino proton signals, indicators of base-pairing and therefore secondary structure, of a double-stranded DNA oligonucleotide in HeLa cells, using selective excitation. We demonstrate the reproducible quantification of in-cell selective longitudinal relaxation times (selT₁), which are reduced

SUBMITTER: Annecke HTP 

PROVIDER: S-EPMC11614993 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature