Noninvasive biomarkers for the detection of GERD-induced pulmonary injury.

ABSTRACT:

Background

The role of gastroesophageal reflux in progressive lung damage is increasingly recognized. We have proposed, based on our work with lung transplant recipients, a novel immune mechanism of pulmonary injury after aspiration of gastric contents, during which higher levels of normally sequestered lung self-antigens (SAgs) collagen V (Col-V) and K-alpha-1 tubulin (Kα1T) in circulating small extracellular vesicles (EVs) induce the production of self-antibodies (SAbs) anti-Col-V and anti-Kα1T. Thus, we aimed to determine whether levels of SAbs or SAgs increased in an animal model of aspiration-induced lung damage in a nontransplant setting.

Methods

We created a murine model of repetitive lung aspiration using C57BL/6J mice. Mice were aspirated weekly with 1 mL/kg of hydr

SUBMITTER: Latorre-Rodriguez AR 

PROVIDER: S-EPMC11615114 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature