Bactogram: Spatial Analysis of Bacterial Colonisation in Epidermal Wounds.

ABSTRACT: Skin barrier damage and subsequent development of harmful microbiota contribute to conditions such as wound infections, atopic dermatitis and chronic wounds, which impact millions of people globally and pose a significant economic burden on healthcare systems. Established microbial sampling methods, such as swabs and tissue biopsies, provide limited information on the spatial distribution of bacteria. We here describe a new method that produces a visual map of the distribution of cultivable bacteria, denoted 'Bactogram', across the whole wound and surrounding skin, suitable for image-based quantification. As part of an exploratory endpoint in a clinical trial we applied the Bactogram method to 48 suction blister wounds in 24 healthy volunteers. Bacteria developed in all wounds, predominant

SUBMITTER: Wallblom K 

PROVIDER: S-EPMC11615128 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature