Structural and genetic basis for the binding of a mouse monoclonal antibody to Flavobacterium psychrophilum lipopolysaccharide.

ABSTRACT: A mouse monoclonal antibody (mAb FL100A) previously prepared against Flavobacterium psychrophilum (Fp) CSF259-93 has now been examined for binding to lipopolysaccharides (LPS) of this strain and Fp 950106-1/1. The corresponding O-polysaccharides (O-PS) of these strains are formed by identical trisaccharide repeats composed of l-Rhamnose (l-Rha), 2-acetamido-2-deoxy-l-fucose (l-FucNAc) and 2-acetamido-4-R₁-2,4-dideoxy-d-quinovose (d-Qui2NAc4NR₁) where R₁ represents a dihydroxyhexanamido moiety. The O-PS loci of these strains are also identical except for the gene (wzy1 or wzy2) that encodes the polysaccharide polymerase. Accordingly, adjacent O-PS repeats are joined through d-Qui2NAc4NR₁ and l-Rha by wzy2-dependent α(1-2) linkages in Fp CSF259-93

SUBMITTER: Cisar JO 

PROVIDER: S-EPMC11615152 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature