Stabilization of expandable DNA repeats by the replication factor Mcm10 promotes cell viability.

ABSTRACT: Trinucleotide repeats, including Friedreich's ataxia (GAA)_n repeats, become pathogenic upon expansions during DNA replication and repair. Here, we show that deficiency of the essential replisome component Mcm10 dramatically elevates (GAA)_n repeat instability in a budding yeast model by loss of proper CMG helicase interaction. Supporting this conclusion, live-cell microscopy experiments reveal increased replication fork stalling at the repeat in mcm10-1 cells. Unexpectedly, the viability of strains containing a single (GAA)₁₀₀ repeat at an essential chromosomal location strongly depends on Mcm10 function and cellular RPA levels. This coincides with Rad9 checkpoint activation, which promotes cell viability, but initiates repeat expansions via DNA synthesis by

SUBMITTER: Masnovo C 

PROVIDER: S-EPMC11615337 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature