Development and validation of the Normalized Organoid Growth Rate (NOGR) metric in brightfield imaging-based assays.

ABSTRACT: This study focuses on refining growth-rate-based drug response metrics for patient-derived tumor organoid screening using brightfield live-cell imaging. Traditional metrics like Normalized Growth Rate Inhibition (GR) and Normalized Drug Response (NDR) have been used to assess organoid responses to anticancer treatments but face limitations in accurately quantifying cytostatic and cytotoxic effects across varying growth rates. Here, we introduce the Normalized Organoid Growth Rate (NOGR) metric, specifically developed for brightfield imaging-based assays. A label-free image analysis model was applied to segment organoids precisely, track their growth rates over time, and classify viable and dead organoids. Testing eleven phenotypically distinct pancreatic cancer organoid models with five ch

SUBMITTER: Deben C 

PROVIDER: S-EPMC11615385 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature