Laboratory development of an RNA quantitative RT-PCR assay reporting in international units for hepatitis D virus.

ABSTRACT:

Introduction

Chronic hepatitis D virus (HDV) is associated with rapid progression to severe liver disease. Co-infection with HDV and hepatitis B virus is likely underdiagnosed due to challenges in diagnostic test availability and standardization. With new HDV antiviral options, HDV RNA quantification is essential for understanding the patient response to treatment. To this end, a quantitative real-time reverse transcription PCR (qRT-PCR) assay utilizing synthetic RNA calibrators and a conversion factor to quantify HDV RNA in WHO international standard units (IU/mL) was developed and validated.

Methods

qRT-PCR primers and probes were selected within the ribozyme region. Thermocycling conditions and reactions were optimized. Synthetic RNA transcripts were prepared as quantific

SUBMITTER: Osiowy C 

PROVIDER: S-EPMC11615724 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature