ASH1L in Hepatoma Cells and Hepatic Stellate Cells Promotes Fibrosis-Associated Hepatocellular Carcinoma by Modulating Tumor-Associated Macrophages.

ABSTRACT: Hepatocellular carcinoma (HCC) often occurs in the context of fibrosis or cirrhosis. Methylation of histone is an important epigenetic mechanism, but it is unclear whether histone methyltransferases are potent targets for fibrosis-associated HCC therapy. ASH1L, an H3K4 methyltransferase, is found at higher levels in activated hepatic stellate cells (HSCs) and hepatoma cells. To determine the role of ASH1L in vivo, transgenic mice with conditional Ash1l depletion in the hepatocyte cell lineage (Ash1l^flox/floxAlb^cre) or HSCs (Ash1l^flox/floxGFAP^creERT2) are generated, and these mice are challenged in a diethylnitrosamine (DEN)/carbon tetrachloride (CCl₄)-induced model of liver fibrosis and HCC. Depleting Ash1l in both hepatocytes and HSCs

SUBMITTER: Du Y 

PROVIDER: S-EPMC11615825 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature