Hydrocortisone, Ascorbic Acid, and Thiamine (HAT) Therapy Decreases Oxidative Stress, Improves Cardiovascular Function, and Improves Survival in Murine Sepsis.

ABSTRACT:

Introduction

A small clinical trial showed HAT therapy improved survival but no studies have been reported in animal models to examine potential mechanisms.

Methods

Sepsis was induced in female mice using the cecal ligation and puncture (CLP) model. Physiologic parameters including heart rate (HR), pulse distension (PD), and respiratory rate (RR) were measured noninvasively at baseline, 6 and 24 h post CLP. These measurements stratified mice into predicted to live (Live-P) or die (Die-P). Mice were randomized to receive HAT therapy or vehicle. Oxidative stress was measured in peritoneal exudative cells 24 h after CLP.

Results

HR, PD, and RR all declined within the first 6 h of sepsis and were significantly lower in the Die-P mice compared with Live-P. HR 6 h post-CLP

SUBMITTER: Kim J 

PROVIDER: S-EPMC11615833 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature