Dynamic Phase Behavior of Amorphous Solid Dispersions Revealed with <i>In Situ</i> Stimulated Raman Scattering Microscopy.

ABSTRACT: This study reports the application of in situ stimulated Raman scattering (SRS) microscopy for real-time chemically specific imaging of dynamic phase phenomena in amorphous solid dispersions (ASDs). Using binary ritonavir and poly(vinylpyrrolidone-vinyl acetate) films with different drug loadings (0-100% w/w) as model systems, we employed SRS microscopy with fast spectral focusing to analyze ASD behavior upon contact with a dissolution medium. Multivariate unmixing of the SRS spectra allowed changes in the distributions of the drug, polymer, and water to be (semi)quantitatively imaged in real time, both in the film and the adjacent dissolution medium. The SRS analyses were further augmented with complementary correlative sum frequency generation and confocal reflection for additiona

SUBMITTER: Tomberg T 

PROVIDER: S-EPMC11615945 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature