Phosphatidylinositol 4,5-bisphosphate drives the formation of EGFR and EphA2 complexes.

ABSTRACT: Receptor tyrosine kinases (RTKs) regulate many cellular functions and are important targets in pharmaceutical development, particularly in cancer treatment. EGFR and EphA2 are two key RTKs that are associated with oncogenic phenotypes. Several studies have reported functional interplay between these receptors, but the mechanism of interaction is still unresolved. Here, we use a time-resolved fluorescence spectroscopy called PIE-FCCS to resolve EGFR and EphA2 interactions in live cells. We tested the role of ligands and found that EGF, but not ephrin A1 (EA1), stimulated heteromultimerization between the receptors. To determine the effect of anionic lipids, we targeted phospholipase C (PLC) activity to alter the abundance of phosphatidylinositol 4,5-bisphosphate (PIP₂). We found

SUBMITTER: Singh PK 

PROVIDER: S-EPMC11616708 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature