Human immunodeficiency virus-1 induces host genomic R-loops and preferentially integrates its genome near the R-loop regions.

ABSTRACT: Although HIV-1 integration sites favor active transcription units in the human genome, high-resolution analysis of individual HIV-1 integration sites has shown that the virus can integrate into a variety of host genomic locations, including non-genic regions. The invisible infection by HIV-1 integrating into non-genic regions, challenging the traditional understanding of HIV-1 integration site selection, is more problematic because they are selected for preservation in the host genome during prolonged antiretroviral therapies. Here, we showed that HIV-1 integrates its viral genome into the vicinity of R-loops, a genomic structure composed of DNA-RNA hybrids. VSV-G-pseudotyped HIV-1 infection initiates the formation of R-loops in both genic and non-genic regions of the host genome and prefe

SUBMITTER: Park K 

PROVIDER: S-EPMC11616997 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature