A streamlined proximity extension assay using POEGMA polymer-coated magnetic beads for enhanced protein detection.

ABSTRACT: The detection of protein biomarkers presenting at low concentrations in biological fluids is essential for disease diagnosis and therapeutic monitoring. While magnetic beads-based solid-phase immunoassays have shown promise in achieving high sensitivity for detecting low-abundance proteins, existing protocols suffer from limitations such as the cumbersome need for bead blocking and washing steps to minimize adsorption of non-specific biomolecules. These extra requirements lead to increased assay complexity and the risk of procedural errors. In this study, we present a streamlined magnetic proximity extension assay (MagPEA) using poly (oligo (ethylene glycol) methacrylate) (POEGMA)-coated beads. The polymer brush on bead surface, on the one hand, provides an effective mechanism for repellin

SUBMITTER: Hu J 

PROVIDER: S-EPMC11617200 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature