Targeted deletion of olfactory receptors in <i>D. melanogaster</i> via CRISPR/Cas9-mediated LexA knock-in.

ABSTRACT: The study of olfaction in Drosophila melanogaster has greatly benefited from genetic reagents such as olfactory receptor mutant lines and GAL4 reporter lines. The CRISPR/Cas9 gene-editing system has been increasingly used to create null receptor mutants or replace coding regions with GAL4 reporters. To further expand this toolkit for manipulating fly olfactory receptor neurons (ORNs), we generated null alleles for 11 different olfactory receptors by using CRISPR/Cas9 to knock in LexA drivers, including multiple lines for receptors which have thus far lacked knock-in mutants. The targeted neuronal types represent a broad range of antennal ORNs from all four morphological sensillum classes. Additionally, we confirmed their loss-of-function phenotypes, assessed receptor haploinsufficie

SUBMITTER: Zhang R 

PROVIDER: S-EPMC11617259 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature